cellgeni/postcode_decoding
subworkflowSort SAM/BAM/CRAM file
spatial transcriptomics decoding postcode probabilistic decoding
Module Information
Components
This subworkflow uses the following modules:
Inputs
| Name | Type | Description |
|---|---|---|
| ch_profile | file | The input channel containing the spot/cell profile files Structure: [ val(meta), path(profile) ] |
| ch_tabular_codebook | file | The codebook in a tabular format Structure: [ val(meta), path(tab_codebook) ] |
| ch_readout_file | file | The input channel containing the readout file for MERFISH-like data Structure: [ val(meta), path(readout) ] |
| ch_R | int | The number of imaging round for the profile Structure: [ val(meta), 6 ] |
| ch_loc | file | The cell/pixel/spot locatisable tsv file Structure: [ val(meta), path(localisation) ] |
Outputs
| Name | Type | Description |
|---|---|---|
| decoding_result | file | Channel containing decoded results Structure: [ val(meta), path(csv) ] |
| processed_profiles | file | Channel containing reformatted profile files Structure: [ val(meta), path(profile) ] |
| versions | file | File containing software versions Structure: [ path(versions.yml) ] |
Quick Start
Include this subworkflow in your Nextflow pipeline:
include { POSTCODE_DECODING } from 'https://github.com/cellgeni/nf-modules/tree/main/subworkflows/sanger-cellgeni/postcode_decoding'