cellgeni/postcode_decoding

subworkflow

Sort SAM/BAM/CRAM file

spatial transcriptomics decoding postcode probabilistic decoding

Module Information

Repository
https://github.com/cellgeni/nf-modules/tree/main/subworkflows/sanger-cellgeni/postcode_decoding
Source
Sanger (cellgeni)
Organization
cellgeni
Authors
@BioinfoTongLI
Maintainers
@BioinfoTongLI

Components

This subworkflow uses the following modules:

Inputs

Name Type Description
ch_profile file The input channel containing the spot/cell profile files Structure: [ val(meta), path(profile) ]
ch_tabular_codebook file The codebook in a tabular format Structure: [ val(meta), path(tab_codebook) ]
ch_readout_file file The input channel containing the readout file for MERFISH-like data Structure: [ val(meta), path(readout) ]
ch_R int The number of imaging round for the profile Structure: [ val(meta), 6 ]
ch_loc file The cell/pixel/spot locatisable tsv file Structure: [ val(meta), path(localisation) ]

Outputs

Name Type Description
decoding_result file Channel containing decoded results Structure: [ val(meta), path(csv) ]
processed_profiles file Channel containing reformatted profile files Structure: [ val(meta), path(profile) ]
versions file File containing software versions Structure: [ path(versions.yml) ]

Quick Start

Include this subworkflow in your Nextflow pipeline:

include { POSTCODE_DECODING } from 'https://github.com/cellgeni/nf-modules/tree/main/subworkflows/sanger-cellgeni/postcode_decoding'
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